Development of a 2D-LC/MS Method for Charge Variant Analysis of ADCs
- Utilize first-dimension cation-exchange chromatography separation coupled online to a 2DLC/MS system to isolate and mass spectrometrically characterize individual charge variants peaks, providing identification of molecular modifications
- Implement a comprehensive, automated 2D-LC/MS platform with minimal manual fraction handling to increase analytical throughput and reproducibility, enabling high-resolution characterization of charge variants