Understanding Bioanalytical Strategies for In Vitro & In Vivo Characterization of Next Generation ADCs

  • Analyze serum aggregation propensity of higher DAR ADCs to measure drug load and identity of novel ADCs, and understand drug loading, structural stability, and early pharmacokinetic clearance
  • Optimize intact high-res mass spec of novel ADCs in biological matrices to monitor average DAR, drug load distribution, and biotransformation
  • Assess a true one-pot assay for ADC quantitation from in vivo studies to streamline bioanalysis and reduce methodological variability